ABSTRACT
Purpose: To assess MTHFR rs 1801133 [C677T] gene polymorphism in diabetic patients as a risk factor for diabetic retinopathy and to establish the changes in Platelet indices and count in diabetic patient as compared to the healthy control group
Patients and Methods: The study included 40 patients with Type 2 Diabetes Mellitus. They were divided into 2 equal groups, 20 patients with Diabetic Retinopathy, 20 patients without Diabetic Retinopathy. Patients were selected from those attending the outpatient Ophthalmology Unit and Diabetes Clinic of Al-Zahraa University Hospital in the period from June 2014 to June 2015. Their ages ranged between 34 to 66 years old
They were 14 males and 26 females. Twenty cases apparently healthy individuals were selected as a control group. All cases were subjected to full history taking and complete ophthalmological examination. Also laboratory investigations were done including complete blood picture, kidney and liver function tests, coagulation profile, urine analysis, lipid profile, fasting and postprandial blood sugar and Genetic study for detection of MTHFR gene C677T mutation [rs 1801133] by real time PCR
Results: In all diabetic patients the mutant homozygous TT showed a highly statistically significant increase in FBS [p=0.000], PPBS [p=0.000], HbAlC [p=0.000] and cholesterol [p=0.001] as compared to wild type. Also in all diabetic patients the mutant homozygous TT showed a highly statistically significant increase in FBS [p=0.002], PPBS [p=0.001], HbAlC [p=0.019] and cholesterol [p=0.012] as compared to heterozygous mutant type
Conclusion: The homozygous mutant type [TT] of rs!801133 was detected in 10% of DR patients group while absent in DWR group and the control group. The heterozygous mutant type [CT] was increased in DR group [50%] as compared to DWR group [35%] and the control group [25%]
Subject(s)
Humans , Female , Male , Adult , Middle Aged , Methylenetetrahydrofolate Reductase (NADPH2) , Polymorphism, Genetic , Diabetes Mellitus, Type 2/genetics , Risk Factors , Blood PlateletsABSTRACT
Plasma fibronectin and serum complement C3 levels were estimated in 30 patients with chronic active hepatitis [CAH] post virus B and another 30 patients with CAH post virus C as well as 20 normal healthy subjects. Fibronectin level was significantly increased in CAH when compared to normal controls. Moreover, fibronectin level was significantly increased in CAH following virus C when compared to CAH following virus B hepatitis. Concerning complement C3 there was no significant changes in the different groups studied. It is concluded that CHA following virus C may lead to vigorous inflammatory damage than CAH following virus B infection